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SAFETY MANUAL   |   APPENDICES

APPENDICES

Safety principles, personal responsibility, safety systems, emergency response and the safety network at IISc.

CHAPTER 11

11.1 Self-Audit Checklist

  1. Complete self-audit and send to Department Safety Champion
  2. Mandatory signage can be downloaded from OLSEH website or the Department Office.
  3. Personal Protective equipment must always be used when working in the laboratory. Check that students are using them.
  4. Lab in-charge is responsible for laboratory safety. Lab users should be quizzed on safety rules during the audit.
  5. Improper chemical segregation is a common issue. Please check the inventory of chemicals to ensure solvents, oxidizers, acids and bases are segregated.
  6. All chemicals must be labelled. Labels can be downloaded from OLSEH website.
  7. All chemical waste must be labelled. Labels can be downloaded from OLSEH website.
  8. Gas cylinders are also often stored improperly and not tagged. Please check. Tags can be downloaded from OLSEH website
  9. Exit doors, including those which open directly from lab spaces into stairwells, cannot be locked during normal business hours. Security measures are allowed after hours, with the approval of the OLSEH office.
  10. Electrical cords that have frayed wires or broken insulation present significant electrical shock and fire hazards. Replace or repair an electrical cord found to be in poor condition.
  11. Cover plates must be installed on all electrical outlets and switches to prevent accidental contact with electrical wires.
Table No. 11.1   Self-Audit Checklist
Type # Safety Point to check Yes No NA Remark
General 1 Working telephone near the door
2 Emergency contacts list near telephone at eye level
3 Hazard sheet is filled and current. The sheets must have been signed in the last 6 months.
4 Users are wearing closed toed shoes in the lab. Some labs ask users to take off shoes. In such cases replacement shoes must be provided. No chappals.
5 No evidence of food/drinks being brought inside lab. No tea/coffee cups. No lunch boxes.
6 The lab should look clean and organized. No accumulated junk, like cardboard boxes, trash, etc.
7 Has the lab in-charge instituted an orientation program that all new users must go through to get access to the lab?
8 At-least one light is connected to UPS?
Important Note: Minimize storage of materials in laboratory premises. For example, paper goods, plastic containers, materials stored in boxes and empty containers.
Fire 9 Two feet of unobstructed walking space around labs
10 Fire extinguishers (appropriate type) in working order
11 Fire extinguishers are stored in proper and visible places
12 Equipment that heat up during operation have adequate ventilation
13 No exposed electrical wires, broken plugs, broken switches, etc.
14 Power strips are of appropriate wattage (for the given load)
15 Large equipment connected to earth
Laser 16 High-power laser (Class 3 and above or invisible) must have warning light outside the door
17 Laser safety glasses, if Class 2 laser of above
18 High-power laser (Class 3 and above or invisible lasers) must have a separate partition with floor to wall barrier
Chemical 19 Chemical containers are labelled properly and contain necessary information. No mystery bottles
20 Chemicals are stored in appropriate cupboards. No open shelfs, no wooden (flammable) cupboards. Large amounts of solvents need exhausted cabinets
21 All chemical storage must have hazardous stickers (e.g. corrosive, oxidizer, toxic, etc)
22 All chemical waste must be clearly labelled. There should be appropriate waste disposal facility for all chemicals stored in the lab. e.g. If you have acids, there must be a way to dispose acids, etc.
23 Personal protective eqpt. (Glasses, gloves, aprons, shoes) must be easily accessible, not inside locked cabinets. For liquid hazards need splash goggles, not glasses. For aggressive chemicals, need thick nitrile gloves not thin examination gloves. No latex gloves. Large amounts of chemicals require face shields
24 If corrosive chemicals are used, eye sprays must be nearby,
25 MSDS of all hazardous chemicals are printed out and stored in a file that is easily accessible
26 Acids, bases, oxidizers, and solvents are segregated, either in different cupboards or with secondary containment
27 All refrigerators used to store chemicals, must have “No food” sign; no food in any fridge in any lab
28 Clearly marking on the dustbins to distinguish lab waste (hazardous) and general waste
29 Chemical work can only be done in hoods with proper ventilation
30 Sharps must be disposed in hard containers
31 Bio waste must be appropriately disposed. Red dustbins for soiled napkins, etc. Blood/cultures can be disposed down the drain but only after hypochlorite treatment (labs should have hypochlorite). Dead bodies must be disposed thru Bio departments.
High
pressure
32 Gas cylinders are properly chained to walls, either at half height or 1/3 + 2/3 height. Cylinders must be stored in a tight formation so that they cannot tip. Carts cannot be used for permanent storage
33 Unused or spare gas cylinders must have valve guards
34 All cylinders must have tags that identify the contents, status (empty/full) and owner
35 All hazardous gas lines must have welded VCR connections; highly dangerous gases must have coaxial connections
36 Hazardous gas cylinders need gas cabinets.
37 CVD reactors must be connected to functional exhausts.
38 Any lab with hazardous gasses must have sensors to detect that gas. The sensor should be functional.
Cryo 39 All labs with cryo liquids must have cryo-rated personal protective equipment. Everyone needs gloves. Large amounts need face shields and aprons also.
40 Cryo cylinders must have safety valves and rupture disks.
41 Cryo liquids can only be handled in rated dewars or flasks.
Electrical
hazard
42 Cover plates in place for outlets and switches
43 No extension cords used
44 Inspect AC filters periodically
Exits 45 Illuminated signs working
46 Paths free from obstructions
47 Emergency exits accessible and marked

Additional Checklist for Chemical Storage and Handling
  1. . Generally, light-duty and wooden cabinets should not be used. Cabinets units should be securely anchored to the wall.
  2. To avoid potential contamination, food should not be stored in refrigerators or freezers designated for chemical storage.
  3. Labelling of cabinets by chemical class (e.g. flammable liquids, acids, oxidizers) is essential if chemical storage is to be segregated to avoid incompatibilities, and to identify storage areas for emergency response personnel.
  4. Highly toxic gases, such as fluorine, phosgene, and many semiconductor gases, should be stored in ventilated cabinets made for this purpose. In the event of a leak or fire, the gas cabinet would contain and exhaust the gas, protecting the laboratory worker from exposure.
  5. Toxic or flammable substances that can be airborne (e.g. gases, vapours, dusts, fumes or mists) should not be used in unventilated areas. In the absence of adequate ventilation, air contaminants can build up to levels that pose health or flammability hazards.
  6. Chemical containers should be clearly labelled with at least a chemical name. The manufacturer’s label is best, as it usually contains a great deal of information about health and physical hazards. When a chemical is transferred from the original container, the new container should be labelled, as possible. Small containers may use other means of identification, such as a code or number system referenced to the user’s lab notebook.
  7. In order to avoid spillage or release of vapors, containers should be closed except when transferring
  8. For optimum performance and containment, a fume hood should have the minimum amount of chemicals or apparatus in it when in use. It is particularly important that the slots or baffles at the back of the hood are unobstructed.
  9. Some chemicals may degrade certain container materials. For example, hydrofluoric is incompatible with glass. Inorganic hydroxides are best stored in polyethylene containers. Some organic solvents will soften plastic.
  10. Chemicals which may react violently or emit hazardous fumes when mixed should not be stored near each other. Examples include oxidizers and flammables, acids and bases.
  11. Corrosive materials can cause severe tissue damage and are particularly injurious to the eye. Storage of corrosive below eye level helps to minimize this risk.
  12. Quantities of chemicals in storage should be consistent with the shortterm needs of the lab. Excessive storage should be avoided.
  13. When transporting chemicals between rooms or buildings, secondary containers, such as bottle carriers, should be used. In the event the container is dropped, bumped or otherwise breaks, the contents would be contained in the bottle carrier, avoiding a spill. Bottle carriers are available in many stockrooms.
  14. Some chemicals, such as ethers or other peroxide-formers, have recommended storage time limits. Chemicals stored beyond their limit date may form explosive peroxides, which may detonate when removing a cap, agitating, dropping, scraping, etc. Upon arrival, these containers should be marked with the date placed in storage and an expiration date based on manufacturer’s recommendations. Many manufacturers include an expiration date on the product label.
  15. To avoid difficult and potentially costly waste disposal problems, a procedure should be in place to assure all materials are labeled and unneeded chemicals disposed of properly
CHAPTER 11

11.2 No Food Label for Lab Refrigerators

Chemical Safety
CHAPTER 11

11.3 Cylinder & Chemical Labels

The individual departments are requested to print and stock them for use

Chemical Safety
Chemical Safety
Chemical Safety
CHAPTER 11

11.4 Chemical Waste Label

The individual departments are requested to print and stock them for use.

Chemical Safety
CHAPTER 11

11.5 Laboratory Hazard Sheet

  1. Make a lab layout using some software and save it as an image file. Insert it in the ‘Room floor plan’ box.
  2. Ensure that you include (in the floor plan)
    • ALL windows and doors
    • Location of wet benches, fume hoods, laser assembly, chemical storage, High-Voltage equipment, compressed cylinders and bio-logical hazards
    • Location of lab telephone and fire extinguishers and preferably light switches
  3. Make use of the personal protective equipment table to specify protective gear like ‘Nitrile gloves’, ‘Laser safety glasses - xyz wavelength’ etc. Please be specific with the type of protective gear.
  4. Use the ‘Description of Lab Hazards’ column to indicate hazardous substances. Reference symbols have been provided with this template document. Next to the symbol, specify the hazard as accurately as feasible. Eg: Laser- Class 4; Compressed cylinder-Silane; Acids - HF acid etc.
  5. Prepare a new document once in 6 months or every time you make major changes such as shifting of equipment or addition of hazards in your lab.
Chemical Safety
Chemical Safety Review & Initials (every 6 months )
Emergency Contact Name Office Telephone
Safety In-charge 1 Student name 1 SF-XY Mobile. No.
Safety In-charge 2 Student name 2 FF-XY Mobile. No.
Faculty Faculty Name TF-XY Mobile. No.
Chemical Safety Room Floor Plan:
Chemical Safety Reference Symbols of Hazards
Description of Hazards
Nitrogen cylinder Nitrogen cylinder
Acids in fume hood Acids in fume hood
Class 3B He-Ne Laser Class 3B
He-Ne Laser
HV power supply equipment HV power supply equipment
Protective Equipment
1 Laser safety glasses
632.8 nm
2 Nitrile gloves
3 Lab shoes
4
5
CHAPTER 11

11.6 Safety Policy

The Indian Institute of Science (IISc) is committed to the health and safety of everybody on both its campuses. The purpose of this safety policy is to establish the responsibility of the IISc’s various stakeholders towards this goal. This policy applies to all the IISc’s activities; including research, teaching, incubation, and external projects. Further, this policy applies to all the employees, students, visitors, and contract workers of the IISc.

The policy is based on the following Directive Principles:

  1. Safety is paramount: The safety of everyone on the IISc’s premises is the primary concern. All activities, including research and teaching, must be conducted without compromising safety.
    Accountability: Any person having access to any equipment or material housed in the Institute campus(es) must ensure their and others’ adherence to the highest standards of safety besides taking personal responsibility for his/her actions and omissions.
  2. Trust but also verify: The Institute recognizes that, as a diverse research campus with more than 40 departments within which a variety of research is being conducted, there is a diversity of hazards and attendant risks. The people responsible for setting up facilities and guiding experiments are expected to be aware of the attendant hazards and risks, and are entrusted with taking necessary safety precautions. The primary role of the Safety Committee is to set safety policies while the role of the Office of Laboratory Safety and Environmental Health (OLSEH) is to implement these policies and monitor compliance. The Institute requires its departments to implement its safety policies in both letter and spirit and reserves the right to verify compliance.
1. Definitions

‘Institute’ refers to the Indian Institute of Science and includes all its extended campuses.

‘Laboratory’ refers to a room or space or field used for research, teaching, measurement, or operations. Often laboratories are fraught with chemical, radiation, biological, radiological or other hazards. For the purposes of this policy, the term also includes the pathways along which material is carried from one laboratory to another, storage areas, and preparatory spaces.

The ‘Office of Laboratory Safety and Environmental Health (OLSEH)’ is an office approved by the Council consisting of Environment Health and Safety (EHS) Officers. Its responsibility is to implement Safety Policies.

‘Safety Committee’ is a Committee consisting of faculty members/staff nominated by the Director to oversee and supervise OLSEH and to formulate Safety Policies for the Institute.

‘Department Safety Champion’ is a designated permanent staff or sufficient authority appointed by the Department Chair (or Centre Chair) to oversee safety in a Department/Centre. Every Department/Centre must have a Safety Champion.

‘Employee’ includes any person employed on the Institute’s premises, either directly or through an agent. It includes anyone working on the premises, whether on remuneration, on a stipend, or voluntarily. It includes temporary and/or contract employees, whose terms of employment are expressed or implied. It includes faculty members, whether tenured or on contract, contract workers, post-doctoral scholars, research associates, probationers, project staff, trainees, and interns.

‘Principal Investigator’ or PI, is an Employee, often a faculty member or scientific staff member, under whose supervision an experiment or any other activity is being undertaken in a laboratory.

‘Users’ includes every person using the laboratory, equipment, research infrastructure and other facilities on the Institute’s premises. Most often, these are employees and students. Users can also be short-term and long-term visitors, with the term applying to foreign exchange students, visiting faculty, collaborators, interns, parent/sponsor institutions, employees of companies, and individuals who temporarily access facilities at the Institute under any arrangement.

‘Contractor’ is an entity or person who renders services, provides work, or supplies contract labour to the Institute. This includes the tiers of sub-contractors who may be employed by the contractor.

‘Incident Report’ is a written account of any emergency evacuation, emergency alarm trigger, injury, or accident that occurs in a Department/Centre. Incident Reports must contain a description of the event, a preliminary investigation into the cause, the prima-facie cause, and recommendations to prevent such incidents in the future.

‘Emergency Response Plan’ a clear and sequential plan that prescribes the actions and actors during an emergency. The plan is drafted by each Department Safety Champion in consultation with OLSEH.

‘Emergency Response Team’ is a team drafted by the Department Safety Champion and tasked with supervising an emergency in that Department/Centre.


2. “Safety Rules” means the rules laid down by OLSEH or the IISc Safety Committee that must be followed by all “Users”. These rules will be listed on the OLSEH website and are subject to revision from time to time. All users must abide by the Safety Policy and Safety Rules and will be responsible for their actions/omissions.

The IISc Safety Committee will consist of faculty, staff, and other personnel as per the relevant norms. The primary role of this committee is to:

  1. Review the IISc’s Safety Policy from time to time and suggest modifications if any are required.
  2. Oversee the activities of OLSEH with respect to the effective implementation of safety policies and advise them from time to time about relevant matters.

3. Office of Laboratory Safety and Environmental Health (OLSEH)

The Institute has established the Office of Laboratory Safety and Environmental Health (OLSEH) to be the primary body responsible for the effective implementation of workplace, environmental, and laboratory safety across the Institute. OLSEH shall report to the Institute Safety Committee. OLSEH will comprise EHS Officers and Senior Personnel. OLSEH will:

  1. Conduct periodic risk assessment of all activities, equipment, buildings, policies, procedures, training, and manuals across the institute. These include but are not limited to, research activities; department buildings; and student housing; contractor and vendor regulations; and waste and effluent treatment.
  2. Take suo motu notice of any safety issue and investigate.
  3. Bring to the notice of the Safety Committee, in the form of a report, any hazards observed during assessments or investigations along with recommendations about how to reduce, eliminate and contain said hazards.
  4. Provide recommendations on safety policies and actions to the Safety Committee. The recommendations can include, but are not limited to, such matters as rules for personal protective gear, standard operation procedures, waste disposal, storage, emergency response, and incident reporting. In extreme cases of non-compliance (as outlined in this Policy), OLSEH can recommend the closure of laboratories/facilities. OLSEH can recommend the scrapping of faulty or obsolete equipment at the request of the PI.
  5. Conduct scheduled and spot audits to ensure that the rules of the Safety Policy prescribed by the Safety Committee are being followed in both letter and spirit across the Institute. To conduct these audits, OLSEH has a right to demand complete and unfettered access to all spaces across the Institute.
  6. Inform the Safety Committee of any breach of any prescribed Safety Policy or Safety Rules by any User on the Institute’s campuses.
  7. Periodically review the Institute’s Safety Policy and submit any proposed changes to the Safety Committee for approval. Once approved, OLSEH will inform all stakeholders of the changes made to the policy. This must be done within a reasonable amount of time.
  8. Implement the directions of the IISc Safety Committee. This may include matters related to technical advice, the implementation of policies, the conducting of investigations, the procurement of information, and follow-up on action items.
  9. Ensure that the Institute has all the required statutory approvals it needs from the state and central governments and other regulatory agencies.
  10. Assist the PI in obtaining required equipment- or facility-specific statutory approvals from the state and central governments and other regulatory agencies.
  11. Serve as the Institute’s primary point of contact with all regulatory agencies and governmental departments.
  12. Facilitate any external safety audits mandated by regulatory agencies or appointed by the Safety Committee from time to time.
  13. Solicit the Safety Committee for funds to improve the safety infrastructure in the institute.
  14. Serve as the primary source of contact for Department Safety Champions (on behalf of Safety Committee).
  15. Compile the biannual self-audit reports from Safety Champions of individual departments.
  16. Maintain a list of Department Safety Champions, along with their emergency contacts.
  17. Maintain a record of all safety incident reports generated by the Department Safety Champions.
  18. Serve notices to Users/PIs in case of violations of this Policy.
  19. Coordinate with campus security, the Health Centre, and the Centre for Campus Management & Development (CCMD) to provide the support necessary for ensuring safety within the Institute’s campuses.

4. Department Safety Champion

Each department or centre shall have a designated Safety Champion, who must be an Employee of the Institute and part of the cadre of faculty and scientific staff with sufficient authority. The Safety Champion will be appointed by the department Chair and OLSEH will be informed of the appointment. The Safety Champion will:

  1. Be the primary point of contact at a Department/Centre for the Safety Committee and OLSEH.
  2. Be responsible for identifying and informing OLSEH about any unrecognized hazards in the department.
  3. Maintain a list of Principal Investigators for all laboratories, along with their emergency contacts.
  4. Maintain records of the biannual safety-audits submitted by each Principal Investigator and compile these biannual self-audits into a report to be submitted to the OLSEH every six months.
  5. Conduct investigation into any safety complaint from users and, if necessary, escalate the issue to the IISc Safety Committee and OLSEH.
  6. Generate and maintain records of Safety Incident Reports. A copy of these must be sent to OLSEH and shared with the department’s members.
  7. Establish an Emergency Response Plan for the department as per the norms set out by the Safety Committee. This plan may include details about fire prevention, fire alarms, emergency evacuation, and assembly points.
  8. Educate the department community about the Emergency Plan in a mandatory safety session and through a surprise emergency drill, to be organized at least once every year.
  9. Establish an emergency response team responsible for overseeing any emergency response. A subset of the ERT must be on call 24x7 to respond to an emergency. The ERT must ensure complete evacuation, offer technical advice to first responders, inform OLSEH, inform the Security Officer, call the Emergency vehicle(s), and manage the assembly points. The ERT is expected to understand the safety hazards present in the Department. The ERT is also the only body with the authority to declare an "all-clear".

5. Principal Investigator

All laboratories must have a supervising ‘Principal Investigator’ or PI. Typically, this will be a faculty member, scientific officer, or senior project staff member. The PI is entrusted with the safety and well-being of all Users and Contractors in their laboratory. The PI will:

  1. Be responsible for implementing the safety policies prescribed by OLSEH in their laboratory. Any sought exceptions must be approved by OLSEH.
  2. Be responsible for arranging funds to implement the safety policies prescribed by OLSEH. This includes, but is not limited to, funds for personal protective equipment, safety detectors, storage cabinets, fume hoods, gas cabinets, signage, first aid boxes, and fire exits.
  3. Appoint a laboratory safety monitor. The monitor could be a senior research student, project staff member or a scientific/technical staff member.
  4. Ensure the prominent display of the laboratory plan that lists hazards and entry/exits using the format specified by OLSEH.
  5. Develop well-documented safety protocols for non-standard equipment/facilities and ensure all users are trained in these protocols.
  6. Respond to any request for safety infrastructure or complaints made by the Users in their laboratory.
  7. Provide a structure to appropriately train Users of their laboratory in order to manage possible laboratory hazards. The structure must be comprehensive and designed with a ‘reasonable’ User in mind.
  8. Monitor the entry of personnel into their labs so that only people with appropriate safety training are exposed to lab hazards. Conversely, people who cannot have such training must be excluded from labs.
  9. Conduct a biannual safety audit of their laboratory. The self-audit should be comprehensive and prepared in the format prescribed by OLSEH.
  10. Submit the following statement to the Registrar for all new projects:
“The risk assessment for these planned activities has been done. Infrastructure to conduct the activity safely either exists in the lab or is built into the funding structure of the current proposal.”

6. Users

All Users will:

  1. Be personally responsible for their own safety.
  2. Follow the Safety Rules prescribed by the Safety Committee and/or PI.
  3. Undergo training in laboratory protocols and emergency responses
  4. Learn about and understand the potential hazards present in their laboratories and act in a way to minimize the risk posed by those hazards.
  5. Not indulge in any activity that, in their own assessment, can pose a threat either to themselves or to others in the Institute premises. In this, they are allowed to override directions given by the PI.
  6. Inform the Department Safety Champion of any concern to violate safety norms. These reports will be kept confidential. In extreme cases, information may need to be provided to the OLSEH or Safety Committee.
  7. Strictly adhere to the safety practices and standard operating procedures established for the laboratories they work in. All Users are expected to be trained and tested in these practices and procedures before starting work in the laboratory to which they are assigned, and must undergo retraining and testing at regular intervals. Serious violations might lead to the exclusion of the Users from the laboratory for a specified period or indefinitely.

7. Consequences of Non-compliance and Indemnity

Any violation of this Safety Policy or Safety Rules will be handled as follows:

  1. For a first-time violation, OLSEH will send a white notice to the responsible Principal Investigator, with a time frame for remedial action based on the severity of the violation.
  2. If the violation is not remedied within the timeframe, OLSEH will send a yellow notice to the responsible Principal Investigator with an insistence on immediate action.
  3. In case of non-compliance, even after the issuing of a yellow notice, OLSEH will send a red notice to the offending Principal Investigator and order a temporary closure of the laboratory/ facility until the violation is remedied.
  4. In cases of severe violations requiring immediate remedial action, the OLSEH office may directly issue a yellow or red notice.
  5. In the event of damage to property or other loss caused to the Institute due to the violation of the Safety Policy or Safety Rules, the User/s shall fully indemnify the Institute and hold the Institute harmless against all loss, injury and damage. In addition, the Institute reserves the right to claim damages/compensation for any loss caused by the actions/ omissions of the User in relation to the Institute’s Safety Policy or Safety Rules.

8. Acknowledgement

The lack of attention to sound safety practices can lead to the loss of property, the compromising of years of painstaking research, serious injury, and, in extreme cases, the loss of life. The Institute, therefore, considers it the duty of all individuals to adhere to this Safety Policy and the attendant Safety Rules published on the OLSEH website.

All faculty, staff, and registered students of the Institute are expected to read and understand this Safety Policy and agree to work towards adopting a strong culture of safety.

Visitors, short-term and long-term users (such as foreign exchange students), visiting faculty, collaborators, interns, employees of companies and individuals who temporarily access facilities at the Institute under any arrangement need to acknowledge the Institute’s safety policy in the manner given below.

Author: OLSEH Team
Last Updated on: 01 October 2026